Attachment and proliferation of fibroblasts and osteoblasts: effects of uniform micro-groove patterns with different widths

doi:10.3969/j.issn.2095-4344.2014.51.009

Abstract

Abstract:

BACKGROUND: The adhesion and proliferation of eukaryotic cells is affected significantly by the microstructure of biocompatible materials.

OBJECTIVE: To investigate the effects of microstructure of titanium surfaces on adhesion and proliferation of osteoblasts and fibroblasts, and explore topography surfaces for implant.

METHODS: Titanium materials with five kinds (100, 50, 20, 10 and 5 µm) of uniform micro-groove surfaces were prepared in 2 µm depth, and smooth titanium plate served as the control group. The roughness of the different titanium sample and surface wettability were detected. The titanium samples of different morphologies were co-cultured with osteoblast MG63 and fibroblasts L929 in vitro.

RESULTS AND CONCLUSION: The surface wettability and surface roughness of micro-grooved titanium groups increased compared with the control group. Effects of different micro-groove surfaces had different effects on the attachment and proliferation of different cells. Uniform micro-groove surfaces generally promoted fibroblast adhesion and osteoblast proliferation (P < 0.05). However, the micro-groove of which scale was similar to cell size had a negative impact on cell behavior.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: biocompatible materials, surface properties, titanium, osteoblasts, fibroblasts

CLC Number:

R318

Mao Fei-fei, Chen Shu-hui, Nie Fei-long, Lu Hui, Chen Jing, Wei Shi-cheng. Attachment and proliferation of fibroblasts and osteoblasts: effects of uniform micro-groove patterns with different widths[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8243-8247.

Figures/Tables 4

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